Benutzer: Gast  Login
Mehr Felder
Einfache Suche
Titel:

PUFA-Induced Metabolic Enteritis as a Fuel for Crohn's Disease.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Schwärzler, Julian; Mayr, Lisa; Vich Vila, Arnau; Grabherr, Felix; Niederreiter, Lukas; Philipp, Maureen; Grander, Christoph; Meyer, Moritz; Jukic, Almina; Tröger, Simone; Enrich, Barbara; Przysiecki, Nicole; Tschurtschenthaler, Markus; Sommer, Felix; Kronberger, Irmgard; Koch, Jakob; Hilbe, Richard; Hess, Michael W; Oberhuber, Georg; Sprung, Susanne; Ran, Qitao; Koch, Robert; Effenberger, Maria; Kaneider, Nicole C; Wieser, Verena; Keller, Markus A; Weersma, Rinse K; Aden, Konrad; Rosenstiel, Ph...     »
Abstract:
BACKGROUND & AIMS: Crohn's disease (CD) globally emerges with Westernization of lifestyle and nutritional habits. However, a specific dietary constituent that comprehensively evokes gut inflammation in human inflammatory bowel diseases remains elusive. We aimed to delineate how increased intake of polyunsaturated fatty acids (PUFAs) in a Western diet, known to impart risk for developing CD, affects gut inflammation and disease course. We hypothesized that the unfolded protein response and antioxidative activity of glutathione peroxidase 4 (GPX4), which are compromised in human CD epithelium, compensates for metabolic perturbation evoked by dietary PUFAs. METHODS: We phenotyped and mechanistically dissected enteritis evoked by a PUFA-enriched Western diet in 2 mouse models exhibiting endoplasmic reticulum (ER) stress consequent to intestinal epithelial cell (IEC)-specific deletion of X-box binding protein 1 (Xbp1) or Gpx4. We translated the findings to human CD epithelial organoids and correlated PUFA intake, as estimated by a dietary questionnaire or stool metabolomics, with clinical disease course in 2 independent CD cohorts. RESULTS: PUFA excess in a Western diet potently induced ER stress, driving enteritis in Xbp1-/-IEC and Gpx4+/-IEC mice. ω-3 and ω-6 PUFAs activated the epithelial endoplasmic reticulum sensor inositol-requiring enzyme 1α (IRE1α) by toll-like receptor 2 (TLR2) sensing of oxidation-specific epitopes. TLR2-controlled IRE1α activity governed PUFA-induced chemokine production and enteritis. In active human CD, ω-3 and ω-6 PUFAs instigated epithelial chemokine expression, and patients displayed a compatible inflammatory stress signature in the serum. Estimated PUFA intake correlated with clinical and biochemical disease activity in a cohort of 160 CD patients, which was similarly demonstrable in an independent metabolomic stool analysis from 199 CD patients. CONCLUSIONS: We provide evidence for the concept of PUFA-induced metabolic gut inflammation which may worsen the course of human CD. Our findings provide a basis for targeted nutritional therapy.
Zeitschriftentitel:
Gastroenterology
Jahr:
2022
Band / Volume:
162
Heft / Issue:
6
Seitenangaben Beitrag:
1690-1704
Volltext / DOI:
doi:10.1053/j.gastro.2022.01.004
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/35031299
Print-ISSN:
0016-5085
TUM Einrichtung:
Lehrstuhl für Translationale Tumorforschung (DKTK) (Prof. Saur)
 BibTeX