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Title:

In vivo neutralization of colchicine toxicity by a PASylated anticalin in a rat model.

Document type:
Journal Article
Author(s):
Jerschke, Elena; Barkovsky, Mikhail; Jung, Nicole; Neuberger, Heidi; Stenzel, Jochen; Eyer, Florian; Skerra, Arne; Geith, Stefanie
Abstract:
We have investigated the pharmacokinetics (PK) and in vivo activity of an Anticalin exhibiting picomolar affinity towards colchicine, a plant toxin with low tolerable dose in humans. PK analysis of the 20-kDa "Colchicalin" protein in male Sprague Dawley rats (n = 3) revealed a very short plasma half-life (3.5 min), which was prolonged 21-fold via genetic fusion with a 200-residue Pro/Ala sequence (PASylation). The scavenging activity of the PASylated Colchicalin was investigated over 3.5 h via s...     »
Journal title abbreviation:
Toxicology
Year:
2023
Journal volume:
492
Fulltext / DOI:
doi:10.1016/j.tox.2023.153526
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/37116682
Print-ISSN:
0300-483X
TUM Institution:
Abteilung für Toxikologie (Prof. Eyer)
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