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Original title:
Precision Design and Characterization of DNA Origami Nanostructures Using High-Resolution Cryo-EM
Translated title:
Präzisionsdesign und Charakterisierung von DNA-Origami-Nanostrukturen mittels hochauflösender Kryo-Elektronenmikroskopie
Author:
Kohler, Fabian
Year:
2021
Document type:
Dissertation
Faculty/School:
Fakultät für Physik
Advisor:
Dietz, Hendrik (Prof. Dr.)
Referee:
Dietz, Hendrik (Prof. Dr.); Simmel, Friedrich C. (Prof. Dr.)
Language:
en
Subject group:
PHY Physik
Keywords:
DNA origami, DNA nanotechnology, cryo-EM, cryogenic electron microscopy
Translated keywords:
DNA origami, DNA Nanotechnologie, Kryo-EM, Kryoelektronenmikroskopie
TUM classification:
PHY 820; TEC 030
Abstract:
To fully exploit the potential of DNA nanostructure design, precise experimental characterization is required. Using cryo electron microscopy methods, we reveal details up to nucleotide resolution and the structural heterogeneity of DNA nanoobjects. We analyze important design elements, including global twist, multi-scaffold design, and DNA crossovers. Moreover, we develop and analyze strategies for the effective fabrication of corners along with the implementation of structural modifications.
Translated abstract:
Um das Potential von DNA-Nanostrukturdesign vollends auszuschöpfen, bedarf es präziser experimenteller Charakterisierung. Mit Hilfe von Kryo-EM Methoden enthüllen wir Details mit bis zu nukleodidgenauer Auflösung sowie strukturelle Heterogenität in DNA-Nanoobjekten. Wir analysieren wichtige Designelemente, darunter globale Torsion, Multi-Scaffold-Design, und DNA-Crossover. Außerdem entwickeln und analysieren wir Strategien zur effektiven Herstellung sowie struktureller Modifikation von Ecken.
WWW:
https://mediatum.ub.tum.de/?id=1616023
Date of submission:
07.07.2021
Oral examination:
24.09.2021
File size:
102288421 bytes
Pages:
193
Urn (citeable URL):
https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20210924-1616023-1-8
Last change:
02.11.2021
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