Skin and gut imprinted helper T cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity.

Hiltensperger, Michael; Beltrán, Eduardo; Kant, Ravi; Tyystjärvi, Sofia; Lepennetier, Gildas; Domínguez Moreno, Helena; Bauer, Isabel J; Grassmann, Simon; Jarosch, Sebastian; Schober, Kilian; Buchholz, Veit R; Kenet, Selin; Gasperi, Christiane; Öllinger, Rupert; Rad, Roland; Muschaweckh, Andreas; Sie, Christopher; Aly, Lilian; Knier, Benjamin; Garg, Garima; Afzali, Ali M; Gerdes, Lisa Ann; Kümpfel, Tania; Franzenburg, Sören; Kawakami, Naoto; Hemmer, Bernhard; Busch, Dirk H; Misgeld, Thomas; Dornmair, Klaus; Korn, Thomas     «

Hiltensperger, Michael; Beltrán, Eduardo; Kant, Ravi; Tyystjärvi, Sofia; Lepennetier, Gildas; Domínguez Moreno, Helena; Bauer, Isabel J; Grassmann, Simon; Jarosch, Sebastian; Schober, Kilian; Buchholz, Veit R; Kenet, Selin; Gasperi, Christiane; Öllinger, Rupert; Rad, Roland; Muschaweckh, Andreas; Sie, Christopher; Aly, Lilian; Knier, Benjamin; Garg, Garima; Afzali, Ali M; Gerdes, Lisa Ann; Kümpfel, Tania; Franzenburg, Sören; Kawakami, Naoto; Hemmer, Bernhard; Busch, Dirk H; Misgeld, Thomas; Dorn...     »

Multidimensional single-cell analyses of T cells have fueled the debate about whether there is extensive plasticity or 'mixed' priming of helper T cell subsets in vivo. Here, we developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of helper T cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system (CNS) in a model of multiple sclerosis (MS) are distinct. i-T cells were Cxcr6+, and m-T cells expressed P2rx7. Notably, m-T cells infiltrated white matter, while i-T cells were also recruited to gray matter. Therefore, we propose that the definition of helper T cell subsets by their site of priming may guide an advanced understanding of helper T cell biology in health and disease.      «

Multidimensional single-cell analyses of T cells have fueled the debate about whether there is extensive plasticity or 'mixed' priming of helper T cell subsets in vivo. Here, we developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of helper T cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show th...     »