Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression.

Flisikowska, Tatiana; Stachowiak, Monika; Xu, Hongen; Wagner, Alexandra; Hernandez-Caceres, Alejandra; Wurmser, Christine; Perleberg, Carolin; Pausch, Hubert; Perkowska, Anna; Fischer, Konrad; Frishman, Dmitrij; Fries, Ruedi; Switonski, Marek; Kind, Alexander; Saur, Dieter; Schnieke, Angelika; Flisikowski, Krzysztof

doi:10.1038/s41598-017-06741-8

2017

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Title:: Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression.
Document type:: Journal Article
Author(s):: Flisikowska, Tatiana; Stachowiak, Monika; Xu, Hongen; Wagner, Alexandra; Hernandez-Caceres, Alejandra; Wurmser, Christine; Perleberg, Carolin; Pausch, Hubert; Perkowska, Anna; Fischer, Konrad; Frishman, Dmitrij; Fries, Ruedi; Switonski, Marek; Kind, Alexander; Saur, Dieter; Schnieke, Angelika; Flisikowski, Krzysztof
Abstract:: We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC truncating mutation orthologous to human APC , analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis. «
We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC truncating mutation orthologous to human APC , analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected d... »
Journal title abbreviation:: Sci Rep
Year:: 2017
Journal volume:: 7
Journal issue:: 1
Pages contribution:: 6613
Fulltext / DOI:: doi:10.1038/s41598-017-06741-8
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/28747659
Print-ISSN:: 2045-2322
TUM Institution:: Lehrstuhl für Experimentelle Tumortherapie (Prof. Saur)
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Institut für Experimentelle Tumortherapie (Prof. Saur)Lehrstuhl für Translationale Tumorforschung (DKTK) (Prof. Saur)2017