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Title:

Tracking stem cell differentiation in the setting of automated optogenetic stimulation.

Document type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Author(s):
Stroh, A; Tsai, HC; Wang, LP; Zhang, F; Kressel, J; Aravanis, A; Santhanam, N; Deisseroth, K; Konnerth, A; Schneider, MB
Abstract:
Membrane depolarization has been shown to play an important role in the neural differentiation of stem cells and in the survival and function of mature neurons. Here, we introduce a microbial opsin into ESCs and develop optogenetic technology for stem cell engineering applications, with an automated system for noninvasive modulation of ESC differentiation employing fast optogenetic control of ion flux. Mouse ESCs were stably transduced with channelrhodopsin-2 (ChR2)-yellow fluorescent protein an...     »
Journal title abbreviation:
Stem Cells
Year:
2011
Journal volume:
29
Journal issue:
1
Pages contribution:
78-88
Language:
eng
Fulltext / DOI:
doi:10.1002/stem.558
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/21280159
Print-ISSN:
1066-5099
TUM Institution:
Fachgebiet Neuroradiologie (Prof. Zimmer); Institut für Neurowissenschaften
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