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Title:

Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Tischbirek, Carsten; Birkner, Antje; Jia, Hongbo; Sakmann, Bert; Konnerth, Arthur
Abstract:
In vivo Ca2+ imaging of neuronal populations in deep cortical layers has remained a major challenge, as the recording depth of two-photon microscopy is limited because of the scattering and absorption of photons in brain tissue. A possible strategy to increase the imaging depth is the use of red-shifted fluorescent dyes, as scattering of photons is reduced at long wavelengths. Here, we tested the red-shifted fluorescent Ca2+ indicator Cal-590 for deep tissue experiments in the mouse cortex in vi...     »
Journal title abbreviation:
Proc Natl Acad Sci U S A
Year:
2015
Journal volume:
112
Journal issue:
36
Pages contribution:
11377-82
Language:
eng
Fulltext / DOI:
doi:10.1073/pnas.1514209112
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/26305966
Print-ISSN:
0027-8424
TUM Institution:
Institut für Neurowissenschaften
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