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Title:

Non-chromatographic preparative purification of enhanced green fluorescent protei

Document type:
Zeitschriftenaufsatz
Author(s):
Hekmat, Dariusch; Maslak, Dominik; Freiherr von Roman, Matthias; Breitschwerdt, Peter; Ströhle, Christoph; Vogt, Alexander; Berensmeier, Sonja; Weuster-Botz, Dirk
Abstract:
Recombinant enhanced green fluorescent protein (eGFP) is used as a marker in numerous applications in biomedical research and diagnostics. For these applications, the macromolecule needs to be provided in a highly purified form. The conventional purification process of eGFP usually consists of multiple subsequent preparative chromatography steps. Since this procedure is costly and time-consuming, an alternative chromatography-free purification process was investigated. This process was a combina...     »
Keywords:
Clarified homogenized fermentation broth; Enhanced green fluorescent protein; Preparative crystallization; Protein purification; Stirred-tank crystallizer
Journal title:
Journal of Biotechnology
Year:
2015
Journal volume:
194
Year / month:
2015-01
Pages contribution:
84-90
Covered by:
Scopus; Web of Science
Reviewed:
ja
Language:
en
Fulltext / DOI:
doi:10.1016/j.chroma.2014.04.063
WWW:
http://www.sciencedirect.com/science/article/pii/S0021967314006530
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