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Title:

Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system

Document type:
Zeitschriftenaufsatz
Author(s):
Frey, S; Haslbeck, M; Hainzl, O; Buchner, J
Abstract:
Antibodies are an important component of the immune system of higher eukaryotes. Furthermore, they are effective tools in basic research, medical diagnostics and therapy. Recombinant expression of these heterotetrameric, disulfide-bridged proteins is usually performed in mammalian cells. Here, we describe the cell-free expression of a mouse monoclonal antibody, MAK33, in a coupled transcription/translation system, based on an Escherichia coli lysate. Both the heavy and the light chain can be pro...     »
Journal title:
Biol.Chem.
Journal title abbreviation:
Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system
Year:
2008
Journal volume:
389
Journal issue:
1
Pages contribution:
37-45
Language:
en
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/18095868
TUM Institution:
Lehrstuhl für Biotechnologie
Format:
Text
Ingested:
16.04.2008
Last change:
21.04.2008
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