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Titel:

Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Frey, S; Haslbeck, M; Hainzl, O; Buchner, J
Abstract:
Antibodies are an important component of the immune system of higher eukaryotes. Furthermore, they are effective tools in basic research, medical diagnostics and therapy. Recombinant expression of these heterotetrameric, disulfide-bridged proteins is usually performed in mammalian cells. Here, we describe the cell-free expression of a mouse monoclonal antibody, MAK33, in a coupled transcription/translation system, based on an Escherichia coli lysate. Both the heavy and the light chain can be pro...     »
Zeitschriftentitel:
Biol.Chem.
Zeitschriftentitel:
Synthesis and characterisation of a functional intact IgG in a prokaryotic cell-free expression system
Jahr:
2008
Band / Volume:
389
Heft / Issue:
1
Seitenangaben Beitrag:
37-45
Sprache:
en
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/18095868
TUM Einrichtung:
Lehrstuhl für Biotechnologie
Format:
Text
Eingabe:
16.04.2008
Letzte Änderung:
21.04.2008
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