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Titel:

Characterization of retroviral vector derived DNA-isoforms by PCR and sequencing

Dokumenttyp:
Article
Autor(en):
Stellberger, Thorsten; Stockmar, Iris; Draxler, Johannes; Dhar, Prabir; Pavlovic, Melanie; Anton, Martina; Koehler, Nina; Dinkelmeier, Anna; Haase, Maren; Schick, Markus; Keller, Ulrich; Busch, Ulrich; Baiker, Armin
Abstract:
For the analytical surveillance of genetic engineering operations suitable methods for characterization of used (recombinant) cell lines and used transfer vector plasmids are needed. The purpose of the described method is the identification of retroviral DNA-isoforms based on lenti-or gammaretrovirus (HIV-1 or MLV). The primers, used in an optimized touch-down PCR protocol, specifically amplify transfer-vector encoded vector genomes and proviruses integrated into stably transduced vertebrate cel...     »
Zeitschriftentitel:
J Verbrauch Lebensm
Jahr:
2019
Band / Volume:
14
Heft / Issue:
2
Seitenangaben Beitrag:
157-165
Volltext / DOI:
doi:10.1007/s00003-019-01215-7
Print-ISSN:
1661-5751
TUM Einrichtung:
III. Medizinische Klinik und Poliklinik (Hämatologie / Onkologie); Institut für Experimentelle Onkologie und Therapieforschung
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