Login
- Title:
Single-Cell Transcriptomics of Human Mesenchymal Stem Cells Reveal Age-Related Cellular Subpopulation Depletion and Impaired Regenerative Function.
- Document type:
- Journal Article
- Author(s):
- Khong, Sacha M L; Lee, Ming; Kosaric, Nina; Khong, Danika M; Dong, Yixiao; Hopfner, Ursula; Aitzetmüller, Matthias M; Duscher, Dominik; Schäfer, Richard; Gurtner, Geoffrey C
- Abstract:
- Although bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely recognized as promising therapeutic agents, the age-related impacts on cellular function remain largely uncharacterized. In this study, we found that BM-MSCs from young donors healed wounds in a xenograft model faster compared with their aged counterparts (p < .001). Given this significant healing advantage, we then used single-cell transcriptomic analysis to provide potential molecular insights into these observations. We found that the young cells contained a higher proportion of cells characterized by a higher expression of genes involved in tissue regeneration. In addition, we identified a unique, quiescent subpopulation that was exclusively present in young donor cells. Together, these findings may explain a novel mechanism for the enhanced healing capacity of young stem cells and may have implications for autologous cell therapy in the extremes of age. Stem Cells 2019; 37:240-246.
- Journal title abbreviation:
- Stem Cells
- Year:
- 2019
- Journal volume:
- 37
- Journal issue:
- 2
- Pages contribution:
- 240-246
- Language:
- eng
- Fulltext / DOI:
- doi:10.1002/stem.2934
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/30412645
- Print-ISSN:
- 1066-5099
- TUM Institution:
- Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
BibTeX