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Titel:

Single-Cell Transcriptomics of Human Mesenchymal Stem Cells Reveal Age-Related Cellular Subpopulation Depletion and Impaired Regenerative Function.

Dokumenttyp:
Journal Article
Autor(en):
Khong, Sacha M L; Lee, Ming; Kosaric, Nina; Khong, Danika M; Dong, Yixiao; Hopfner, Ursula; Aitzetmüller, Matthias M; Duscher, Dominik; Schäfer, Richard; Gurtner, Geoffrey C
Abstract:
Although bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely recognized as promising therapeutic agents, the age-related impacts on cellular function remain largely uncharacterized. In this study, we found that BM-MSCs from young donors healed wounds in a xenograft model faster compared with their aged counterparts (p < .001). Given this significant healing advantage, we then used single-cell transcriptomic analysis to provide potential molecular insights into these observations. We found that the young cells contained a higher proportion of cells characterized by a higher expression of genes involved in tissue regeneration. In addition, we identified a unique, quiescent subpopulation that was exclusively present in young donor cells. Together, these findings may explain a novel mechanism for the enhanced healing capacity of young stem cells and may have implications for autologous cell therapy in the extremes of age. Stem Cells 2019; 37:240-246.
Zeitschriftentitel:
Stem Cells
Jahr:
2019
Band / Volume:
37
Heft / Issue:
2
Seitenangaben Beitrag:
240-246
Sprache:
eng
Volltext / DOI:
doi:10.1002/stem.2934
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/30412645
Print-ISSN:
1066-5099
TUM Einrichtung:
Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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