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Title:

Reverse Transcription Quantitative Polymerase Chain Reaction for Detection of and Differentiation Between RNA and DNA of HIV-1-Based Lentiviral Vectors.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Pavlovic, Melanie; Koehler, Nina; Anton, Martina; Dinkelmeier, Anna; Haase, Maren; Stellberger, Thorsten; Busch, Ulrich; Baiker, Armin E
Abstract:
The purpose of the described method is the detection of and differentiation between RNA and DNA of human immunodeficiency virus (HIV)-derived lentiviral vectors (LV) in cell culture supernatants and swab samples. For the analytical surveillance of genetic engineering, operations methods for the detection of the HIV-1-based LV generations are required. Furthermore, for research issues, it is important to prove the absence of LV particles for downgrading experimental settings in terms of the biosa...     »
Journal title abbreviation:
Hum Gene Ther Methods
Year:
2017
Journal volume:
28
Journal issue:
4
Pages contribution:
215-221
Language:
eng
Fulltext / DOI:
doi:10.1089/hgtb.2017-081
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/28817345
Print-ISSN:
1946-6536
TUM Institution:
Institut für Experimentelle Onkologie und Therapieforschung
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