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Titel:

Evaluation of promoter sequences for the secretory production of a Clostridium thermocellum cellulase in Paenibacillus polymyxa

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Heinze, Simon; Zimmermann, Kerstin; Ludwig, Christina; Heinzlmeir, Stephanie; Schwarz, Wolfgang H.; Zverlov, Vladimir V.; Liebl, Wolfgang; Kornberger, Petra
Abstract:
Due to their high secretion capacity, Gram-positive bacteria from the genus Bacillus are important expression hosts for the high-yield production of enzymes in industrial biotechnology; however, to date, strains from only few Bacillus species are used for enzyme production at industrial scale. Herein, we introduce Paenibacillus polymyxa DSM 292, a member of a different genus, as a novel host for secretory protein production. The model gene cel8A from Clostridium thermocellum was chosen as an ea...     »
Stichworte:
BayBioMS; Cel8A; Heterologous expression; Medium optimization; Secretome analysis; Signal peptide; Strain development
Zeitschriftentitel:
Applied Microbiology and Biotechnology
Jahr:
2018
Band / Volume:
102
Heft / Issue:
23
Seitenangaben Beitrag:
10147-10159
Volltext / DOI:
doi:10.1007/s00253-018-9369-7
Verlag / Institution:
Springer Nature America, Inc
E-ISSN:
0175-75981432-0614
Publikationsdatum:
26.09.2018
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