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Titel:

Detection of the formyl radical by EPR spin-trapping and mass spectrometry

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Bauer, Norbert A.; Hoque, Enamul; Wolf, Manfred; Kleigrewe, Karin; Hofmann, Thomas
Abstract:
For the first time we here present the unambiguous identification of the formyl radical (●CHO) by EPR (Electron Paramagnetic Resonance) spectroscopy and mass spectrometry (MS) using DMPO (5,5-dimethyl-1-pyrroline N-oxide) as spin trap at ambient temperature without using any catalysator(s). The ●CHO was continuously generated by UV photolysis in closed anoxic environment from pure formaldehyde (HCHO) in aqueous solution. The isotropic hyperfine structure constants of ●CHO were determined as aN =...     »
Stichworte:
Ambient temperature; DMPO; EPR; Formyl radical; Mass spectrometry; Simulation
Zeitschriftentitel:
Free Radical Biology and Medicine
Jahr:
2018
Volltext / DOI:
doi:10.1016/j.freeradbiomed.2018.01.002
Verlag / Institution:
Elsevier BV
E-ISSN:
0891-5849
Publikationsdatum:
01.01.2018
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