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Titel:

Evaluation of Kinase Activity Profiling Using Chemical Proteomics

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Ruprecht, Benjamin; Zecha, Jana; Heinzlmeir, Stephanie; Médard, Guillaume; Lemeer, Simone; Kuster, Bernhard
Abstract:
Protein kinases are important mediators of intracellular signaling and are reversibly activated by phosphorylation. Immobilized kinase inhibitors can be used to enrich these often low-abundance proteins, to identify targets of kinase inhibitors, or to probe their selectivity. It has been suggested that the binding of kinases to affinity beads reflects a kinase's activation status, a concept that is under considerable debate. To assess the merits of the idea, we performed a series of experiments...     »
Stichworte:
BayBioMS
Zeitschriftentitel:
ACS Chemical Biology
Jahr:
2015
Band / Volume:
10
Heft / Issue:
12
Seitenangaben Beitrag:
2743-2752
Volltext / DOI:
doi:10.1021/acschembio.5b00616
Verlag / Institution:
American Chemical Society (ACS)
E-ISSN:
1554-89291554-8937
Publikationsdatum:
18.12.2015
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