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Title:

Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A

Document type:
Zeitschriftenaufsatz
Author(s):
Hoermann, Bernhard; Kokot, Thomas; Helm, Dominic; Heinzlmeir, Stephanie; Chojnacki, Jeremy E.; Schubert, Thomas; Ludwig, Christina; Berteotti, Anna; Kurzawa, Nils; Kuster, Bernhard; Savitski, Mikhail M.; Köhn, Maja
Abstract:
The phosphatases PP1 and PP2A are responsible for the majority of dephosphorylation reactions on phosphoserine (pSer) and phosphothreonine (pThr), and are involved in virtually all cellular processes and numerous diseases. The catalytic subunits exist in cells in form of holoenzymes, which impart substrate specificity. The contribution of the catalytic subunits to the recognition of substrates is unclear. By developing a phosphopeptide library approach and a phosphoproteomic assay, we demonstrat...     »
Keywords:
BayBioMS; Hydrolases; Mass spectrometry; Peptides; Phosphorylation; Proteomics
Journal title:
Nature Communications
Year:
2020
Journal volume:
11
Journal issue:
1
Fulltext / DOI:
doi:10.1038/s41467-020-17334-x
Publisher:
Springer Science and Business Media LLC
E-ISSN:
2041-1723
Date of publication:
17.07.2020
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