Identification and confirmation of increased fibrinopeptide a serum protein levels in gastric cancer sera by magnet bead assisted MALDI-TOF mass spectrometry.

Ebert, MP; Niemeyer, D; Deininger, SO; Wex, T; Knippig, C; Hoffmann, J; Sauer, J; Albrecht, W; Malfertheiner, P; Röcken, C

doi:10.1021/pr060011c

2006

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Dokumenttyp:: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):: Ebert, MP; Niemeyer, D; Deininger, SO; Wex, T; Knippig, C; Hoffmann, J; Sauer, J; Albrecht, W; Malfertheiner, P; Röcken, C
Titel:: Identification and confirmation of increased fibrinopeptide a serum protein levels in gastric cancer sera by magnet bead assisted MALDI-TOF mass spectrometry.
Abstract:: Gastric cancer is the second most common malignancy and prognosis remains dismal. The reasons for the poor prognosis are the lack of sensitive serum markers for early detection and screening of high-risk individuals as well as the limited treatment options in advanced cancer stages. Using MALDI-TOF mass spectrometry after prefractionation of sera with magnet hydrophobic C8 coated beads sera from 14 patients with gastric cancer and 14 healthy controls mass spectra were generated. A peptide fragment was found to be highly elevated in cancer sera and was identified as fibrinopeptide A. To confirm proteome analysis of gastric cancer sera, we then screened a larger series of patients with gastric cancer (n = 99), high-risk individuals (n = 13) and normal controls (n = 111) for fibrinopeptide A serum levels. Interestingly, the mean logarithmic concentrations of serum fibrinopeptide A levels were significantly higher in cancer patients (mean 3.636 +/- 0.3738; p < 0.0001) and high-risk individuals (mean 3.569 +/- 0.4722; p < 0.05) compared to normal controls (mean 3.303 +/- 0.4012). In contrast, we observed no association of fibrinopeptide A levels with tumor stage, tumor location, presence of regional or distant metastasis, and Lauren type of gastric cancer. In conclusion, MALDI-TOF mass spectrometry of prefractionated gastric cancer sera allows the identification of potential biomarkers that may lead to the development of serum based tests for screening of high-risk individuals.
Zeitschriftentitel:: J Proteome Res
Jahr:: 2006
Band / Volume:: 5
Heft / Issue:: 9
Seitenangaben Beitrag:: 2152-8
Sprache:: eng
Volltext / DOI:: doi:10.1021/pr060011c
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/16944926
Print-ISSN:: 1535-3893
TUM Einrichtung:: II. Medizinische Klinik und Poliklinik (Gastroenterologie)
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Innere Medizin II, Gastroenterologie (Prof. Schmid)2006