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Title:

Polychrome labeling of bone with seven different fluorochromes: enhancing fluorochrome discrimination by spectral image analysis.

Document type:
Journal Article; Article
Author(s):
Pautke, C; Vogt, S; Tischer, T; Wexel, G; Deppe, H; Milz, S; Schieker, M; Kolk, A
Abstract:
Bone formation and remodeling in vivo can be assessed by polychrome labeling using calcium-binding fluorescent dyes. The number of fluorochromes, however, limits this technique due to the fact that with increasing number, fluorescent spectra inevitably overlap, which makes discrimination more difficult. In order to enhance discrimination, we performed spectral image analysis. Non-critical size defects of the femur of male Wistar rats served as a model for bone formation. Eight different fluoroch...     »
Journal title abbreviation:
Bone
Year:
2005
Journal volume:
37
Journal issue:
4
Pages contribution:
441-5
Language:
eng
Fulltext / DOI:
doi:10.1016/j.bone.2005.05.008
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/16046209
Print-ISSN:
8756-3282
TUM Institution:
Fachgebiet Sportorthopädie (Prof. Imhoff); Institut für Experimentelle Onkologie und Therapieforschung; Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie
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