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- Title:
Establishment of a new murine elastase-induced aneurysm model combined with transplantation.
- Document type:
- Journal Article; Research Support, Non-U.S. Gov't
- Author(s):
- Rowinska, Zuzanna; Gorressen, Simone; Merx, Marc W; Koeppel, Thomas A; Liehn, Elisa A; Zernecke, Alma
- Abstract:
- The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation.Adult male mice (n = 6-9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting.Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared to control grafted mice at 7 week after surgery (1.11 ± 0.10 mm vs. 0.75 ± 0.03 mm; p <= 0,001). Histopathological examination revealed disruption of medial elastin, an increase in collagen content and smooth muscle cells, and neointima formation in aneurysm grafts.We developed a reproducible murine model of elastase-induced aneurysm combined with aortic transplantation. This model may be suitable to investigate aneurysm-specific inflammatory processes and for use in gene-targeted animals.
- Journal title abbreviation:
- PLoS ONE
- Year:
- 2014
- Journal volume:
- 9
- Journal issue:
- 7
- Pages contribution:
- e102648
- Language:
- eng
- Fulltext / DOI:
- doi:10.1371/journal.pone.0102648
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/25068788
- Print-ISSN:
- 1932-6203
- TUM Institution:
- Fachgebiet Gefäßchirurgie (Prof. Eckstein)
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