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Title:

Panoptic vDISCO imaging reveals neuronal connectivity, remote trauma effects and meningeal vessels in intact transparent mice

Document type:
Zeitschriftenaufsatz
Author(s):
Cai, Ruiyao; Pan, Chenchen; Ghasemigharagoz, Alireza; Todorov, Mihail I.; Förstera, Benjamin; Zhao, Shan; Bhatia, Harsharan S.; Mrowka, Leander; Theodorou, Delphine; Rempfler, Markus; Xavier, Anna; Kress, Benjamin T.; Benakis, Corinne; Liesz, Arthur; Menze, Bjoern; Kerschensteiner, Martin; Nedergaard, Maiken; Ertürk, Ali
Abstract:
Analysis of entire transparent rodent bodies could provide holistic information on biological systems in health and disease. However, it has been challenging to reliably image and quantify signal from endogenously expressed fluorescent proteins in large cleared mouse bodies due to the low signal contrast. Here, we devised a pressure driven, nanobody based whole-body immunolabeling technology to enhance the signal of fluorescent proteins by up to two orders of magnitude. This allowed us to image...     »
Journal title:
bioRxiv
Year:
2018
Fulltext / DOI:
doi:10.1101/374785
WWW:
https://www.biorxiv.org/content/early/2018/07/23/374785
Publisher:
Cold Spring Harbor Laboratory
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