To address the accurate structural interpretation of cryogenic electron microscopy maps of DNA nanostructures we develop semi-automated protocols for molecular dynamics-based atomic model building. These models enable annotation and contextualization of the electron density, targeted inspection of isolated design motifs, structural comparison of design iterations, reproducible quantification of global object properties, and provide a reference for the validation of structure prediction models.      «

To address the accurate structural interpretation of cryogenic electron microscopy maps of DNA nanostructures we develop semi-automated protocols for molecular dynamics-based atomic model building. These models enable annotation and contextualization of the electron density, targeted inspection of isolated design motifs, structural comparison of design iterations, reproducible quantification of global object properties, and provide a reference for the validation of structure prediction models....     »