The development of efficient processes for the enzymatic biomass hydrolysis requires a large number of experimental studies. A new impeller was designed and subsequently characterized to make use of a parallel stirred-tank reactor system on a milliliter-scale. Scale-up of enzymatic hydrolysis processes to the liter-scale was shown to be possible if the same mixing numbers are applied. This was successfully demonstrated with enzymatic hydrolyses of microcrystalline cellulose, wheat straw and granulated corn stover with a solid content of up to 20 % and particle sizes in the millimeter range. After optimization of the reaction conditions for hydrolysis making use of the parallel operated stirred-tank reactors 40 % (w/w) of alkaline pretreated corn stover was released as glucose and 30 % (w/w) as xylose within 24 h. With final sugar concentrations of more than 120 g/L a subsequent usage as substrate for fermentation processes is feasible.
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The development of efficient processes for the enzymatic biomass hydrolysis requires a large number of experimental studies. A new impeller was designed and subsequently characterized to make use of a parallel stirred-tank reactor system on a milliliter-scale. Scale-up of enzymatic hydrolysis processes to the liter-scale was shown to be possible if the same mixing numbers are applied. This was successfully demonstrated with enzymatic hydrolyses of microcrystalline cellulose, wheat straw and gran...
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