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Title:

In vivo fluorescent labeling and tracking of extracellular matrix.

Document type:
Journal Article; Review; Research Support, Non-U.S. Gov't
Author(s):
Fischer, Adrian; Correa-Gallegos, Donovan; Wannemacher, Juliane; Christ, Simon; Machens, Hans-Günther; Rinkevich, Yuval
Abstract:
Connective tissues are essential building blocks for organ development, repair and regeneration. However, we are at the early stages of understanding connective tissue dynamics. Here, we detail a method that enables in vivo fate mapping of organ extracellular matrix (ECM) by taking advantage of a crosslinking chemical reaction between amine groups and N-hydroxysuccinimide esters. This methodology enables robust labeling of ECM proteins, which complement previous affinity-based single-protein met...     »
Journal title abbreviation:
Nat Protoc
Year:
2023
Journal volume:
18
Journal issue:
10
Pages contribution:
2876-2890
Fulltext / DOI:
doi:10.1038/s41596-023-00867-y
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/37558896
Print-ISSN:
1754-2189
TUM Institution:
Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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