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Titel:

High Efficiency Low Cost Fibroblast Nucleofection for GMP Compatible Cell-based Gene Therapy.

Dokumenttyp:
Journal Article
Autor(en):
Zhang, Ziyang; Slobodianski, Alex; Arnold, Astrid; Nehlsen, Jessica; Hopfner, Ursula; Schilling, Arndt F; Perisic, Tatjana; Machens, Hans-Günther
Abstract:
Dermal fibroblast is a powerful tool for the study ofDNA delivery in development of both cell therapy and tissue engineering products. Using genetic modification, fibroblasts can be diversely adapted and made suitable for clinical gene therapy. In this study, we first compared several non-viral transfection methods including nucleofection in rat and human primary dermal fibroblast. In addition, the original protocol for nucleofection of primary mammalian fibroblasts was modi...     »
Zeitschriftentitel:
Int J Med Sci
Jahr:
2017
Band / Volume:
14
Heft / Issue:
9
Seitenangaben Beitrag:
798-803
Sprache:
eng
Volltext / DOI:
doi:10.7150/ijms.19241
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/28824316
TUM Einrichtung:
Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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