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- Original title:
- Precision Design and Characterization of DNA Origami Nanostructures Using High-Resolution Cryo-EM
- Translated title:
- Präzisionsdesign und Charakterisierung von DNA-Origami-Nanostrukturen mittels hochauflösender Kryo-Elektronenmikroskopie
- Author:
- Kohler, Fabian
- Year:
- 2021
- Document type:
- Dissertation
- Faculty/School:
- Fakultät für Physik
- Advisor:
- Dietz, Hendrik (Prof. Dr.)
- Referee:
- Dietz, Hendrik (Prof. Dr.); Simmel, Friedrich C. (Prof. Dr.)
- Language:
- en
- Subject group:
- PHY Physik
- Keywords:
- DNA origami, DNA nanotechnology, cryo-EM, cryogenic electron microscopy
- Translated keywords:
- DNA origami, DNA Nanotechnologie, Kryo-EM, Kryoelektronenmikroskopie
- TUM classification:
- PHY 820; TEC 030
- Abstract:
- To fully exploit the potential of DNA nanostructure design, precise experimental characterization is required. Using cryo electron microscopy methods, we reveal details up to nucleotide resolution and the structural heterogeneity of DNA nanoobjects. We analyze important design elements, including global twist, multi-scaffold design, and DNA crossovers. Moreover, we develop and analyze strategies for the effective fabrication of corners along with the implementation of structural modifications.
- Translated abstract:
- Um das Potential von DNA-Nanostrukturdesign vollends auszuschöpfen, bedarf es präziser experimenteller Charakterisierung. Mit Hilfe von Kryo-EM Methoden enthüllen wir Details mit bis zu nukleodidgenauer Auflösung sowie strukturelle Heterogenität in DNA-Nanoobjekten. Wir analysieren wichtige Designelemente, darunter globale Torsion, Multi-Scaffold-Design, und DNA-Crossover. Außerdem entwickeln und analysieren wir Strategien zur effektiven Herstellung sowie struktureller Modifikation von Ecken.
- WWW:
- https://mediatum.ub.tum.de/?id=1616023
- Date of submission:
- 07.07.2021
- Oral examination:
- 24.09.2021
- File size:
- 102288421 bytes
- Pages:
- 193
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20210924-1616023-1-8
- Last change:
- 02.11.2021
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