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Title:

Evaluation and optimization of hepatocyte culture media factors by design of experiments (DoE) methodology.

Document type:
Journal Article; Article
Author(s):
Dong, J; Mandenius, CF; Lübberstedt, M; Urbaniak, T; Nüssler, AK; Knobeloch, D; Gerlach, JC; Zeilinger, K
Abstract:
Optimization of cell culture media based on statistical experimental design methodology is a widely used approach for improving cultivation conditions. We applied this methodology to refine the composition of an established culture medium for growth of a human hepatoma cell line, C3A. A selection of growth factors and nutrient supplements were systematically screened according to standard design of experiments (DoE) procedures. The results of the screening indicated that the medium additives hep...     »
Journal title abbreviation:
Cytotechnology
Year:
2008
Journal volume:
57
Journal issue:
3
Pages contribution:
251-61
Fulltext / DOI:
doi:10.1007/s10616-008-9168-6
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/19003182
Print-ISSN:
0920-9069
TUM Institution:
Klinik und Poliklinik für Unfallchirurgie
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