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Title:

Maintenance of adult porcine retina and retinal pigment epithelium in perfusion culture: characterisation of an organotypic in vitro model.

Document type:
Journal Article
Author(s):
Kobuch, K; Herrmann, WA; Framme, C; Sachs, HG; Gabel, VP; Hillenkamp, J
Abstract:
The purpose of this study was to characterise an ex-vivo adult porcine retina-retinal pigment epithelium (RPE) perfusion organ culture model. Fresh porcine full-thickness retina-RPE-choroid tissue samples were clamped into tissue carriers and mounted in two-compartment containers. The retinal and choroidal sides were continuously perfused with culture medium. pO(2), [Na(+)], [K(+)], [Cl(-)], [glucose], [lactate], and pH were measured in the medium. Tissue samples were examined after 24h, 4, 7, a...     »
Journal title abbreviation:
Exp Eye Res
Year:
2008
Journal volume:
86
Journal issue:
4
Pages contribution:
661-8
Language:
eng
Fulltext / DOI:
doi:10.1016/j.exer.2008.01.011
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/18295758
Print-ISSN:
0014-4835
TUM Institution:
Klinik und Poliklinik für Augenheilkunde
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