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Title:

Insertion of a FLAG-tag sequence at the end of exon 9 of the TBX5 gene in three induced pluripotent stem cell lines (DHMi004-A-4, DHMi004-A-5, DHMi004-A-6) by CRISPR/Cas9 technology.

Document type:
Journal Article
Author(s):
Lahm, Harald; Singh, Niraj K; Gottmann, Irina; Doppler, Stefanie A; Dzilic, Elda; Preisler, Heike; Schneider, Stephanie; Lange, Rüdiger; Krane, Markus; Dreßen, Martina
Abstract:
The identification of TBX5-related regulatory sequences in genes essential for heart development is hampered by the absence of antibodies which allow precipitation of TBX5:DNA complexes. Employing CRISPR/Cas9 technology, we have inserted a FLAG-tag sequence at the end of exon 9 of the TBX5 gene prior to the stop codon by homologous recombination. The translated TBX5-FLAG fusion protein of the three iPSC lines can effectively be precipitated by anti-FLAG antibodies and, thus, allow the detection...     »
Journal title abbreviation:
Stem Cell Res
Year:
2024
Journal volume:
74
Fulltext / DOI:
doi:10.1016/j.scr.2023.103261
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/38100916
Print-ISSN:
1873-5061
TUM Institution:
Klinik für Herz- und Gefäßchirurgie (DHM) (Prof. Krane)
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