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Titel:

Authentication of Primary Murine Cell Lines by a Microfluidics-Based Lab-On-Chip System.

Dokumenttyp:
Journal Article
Autor(en):
Hong, Yingfen; Singh, Nikita; Bamopoulos, Stefanos; Gjerga, Enio; Schmalbrock, Laura K; Balabanian, Karl; Schick, Markus; Keller, Ulrich; Wirth, Matthias
Abstract:
The reliable authentication of cell lines is a prerequisite for the reproducibility and replicability of experiments. A common method of cell line authentication is the fragment length analysis (FLA) of short-tandem repeats (STR) by capillary electrophoresis. However, this technique is not always accessible and is often costly. Using a microfluidic electrophoresis system, we analyzed the quality and integrity of different murine cell lines by STR profiling. As a proof of concept, we isolated and...     »
Zeitschriftentitel:
Biomedicines
Jahr:
2020
Band / Volume:
8
Heft / Issue:
12
Volltext / DOI:
doi:10.3390/biomedicines8120590
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/33317212
TUM Einrichtung:
III. Medizinische Klinik und Poliklinik (Hämatologie / Onkologie)
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