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Title:

Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life.

Document type:
Article; Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Trepotec, Zeljka; Geiger, Johannes; Plank, Christian; Aneja, Manish K; Rudolph, Carsten
Abstract:
Extensive research in the past decade has brought mRNA closer to the clinical realization of its therapeutic potential. One common structural feature for all cellular messenger RNAs is a poly(A) tail, which can either be brought in cotranscriptionally via the DNA template (plasmid- or PCR-based) or added to the mRNA in a post-transcriptional enzymatic process. Plasmids containing poly(A) regions recombine in E. coli, resulting in extensive shortening of the poly(A) tail. Using a segmented poly(A...     »
Journal title abbreviation:
RNA
Year:
2019
Journal volume:
25
Journal issue:
4
Pages contribution:
507-518
Fulltext / DOI:
doi:10.1261/rna.069286.118
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/30647100
Print-ISSN:
1355-8382
TUM Institution:
Institut für Experimentelle Onkologie und Therapieforschung
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