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Title:

Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Hofmann, Julian; Gadjalova, Iana; Mishra, Ritu; Ruland, Jürgen; Keppler, Selina J
Abstract:
Spatial information of cells in their tissue microenvironment is necessary to understand the complexity of pathophysiological processes. Volumetric imaging of cleared organs provides this information; however, current protocols are often elaborate, expensive, and organ specific. We developed a simplified, cost-effective, non-hazardous approach for efficient tissue clearing and multi-organ volumetric imaging (EMOVI). EMOVI enabled multiplexed antibody-based immunolabeling, provided adequate tiss...     »
Journal title abbreviation:
Front Immunol
Year:
2020
Journal volume:
11
Fulltext / DOI:
doi:10.3389/fimmu.2020.599495
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/33569052
Print-ISSN:
1664-3224
TUM Institution:
Institut für Klinische Chemie und Pathobiochemie
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