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- Document type:
- Article; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
- Author(s):
- Grünewald, Julian; Miller, Bret R; Szalay, Regan N; Cabeceiras, Peter K; Woodilla, Christopher J; Holtz, Eliza Jane B; Petri, Karl; Joung, J Keith
- Title:
- Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
- Abstract:
- The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing.
- Journal title abbreviation:
- Nat Biotechnol
- Year:
- 2023
- Journal volume:
- 41
- Journal issue:
- 3
- Pages contribution:
- 337-343
- Fulltext / DOI:
- doi:10.1038/s41587-022-01473-1
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/36163548
- Print-ISSN:
- 1087-0156
- TUM Institution:
- Klinik und Poliklinik für Innere Medizin I, Kardiologie
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