Engineered CRISPR prime editors with compact, untethered reverse transcriptases.

Dokumenttyp:: Article; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Autor(en):: Grünewald, Julian; Miller, Bret R; Szalay, Regan N; Cabeceiras, Peter K; Woodilla, Christopher J; Holtz, Eliza Jane B; Petri, Karl; Joung, J Keith
Titel:: Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
Abstract:: The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing.
Zeitschriftentitel:: Nat Biotechnol
Jahr:: 2023
Band / Volume:: 41
Heft / Issue:: 3
Seitenangaben Beitrag:: 337-343
Volltext / DOI:: doi:10.1038/s41587-022-01473-1
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/36163548
Print-ISSN:: 1087-0156
TUM Einrichtung:: Klinik und Poliklinik für Innere Medizin I, Kardiologie
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