We investigated the possible role of different substances that could play a role in the regeneration of motor neurons. We used the animal model of a group of gene-deficient mice in comparison to there wild type. Each group was missing one of the substances. After facial axatomy we investigated the cellular changes of regeneration of the early phase on day 1 to 4, the middle and the late phase on day 7 up to day 14 using different techniques of immunohistochemistry.
Interleukin 6 deficient mice showed a clearly reduced migration of lymphocytes into the facial nucleus in the early and in the late phase of regeneration. We could observe less activated microglia and astroglia in the early and in the late phase. There was to be seen an increase in the intensity of Galanin in the sprouting growth cones and a higher number of those. Interleukin 6 deficiency led to a slightly slower regeneration of the Galanin- and the CGRP positive axons. CGRP deficiency led to more migrating lymphocytes into the facial nucleus. eNOS deficiency showed more Galanin positive sprouting growth cones on day 4 and an increased intensity of the Galanin positive neurons. nNOS deficiency led to a reduced migration of lymphocytes in the early and in the late phase. However Galanin deficiency, which is highly expressed after facial axatomy in the facial nucleus in the wild type mice, did not show any effect after trauma.
Conclusion: Surprisingly, after facial axatomy there was no effect on the changes of regeneration in Galanin deficient mice. There was a mild effect of CGRP, eNOS and nNOS deficiency on the intercellular changes and their underlying molecular communication and a strong effect of Interleukin 6 deficiency.
«
We investigated the possible role of different substances that could play a role in the regeneration of motor neurons. We used the animal model of a group of gene-deficient mice in comparison to there wild type. Each group was missing one of the substances. After facial axatomy we investigated the cellular changes of regeneration of the early phase on day 1 to 4, the middle and the late phase on day 7 up to day 14 using different techniques of immunohistochemistry.
Interleukin 6 deficient mice...
»