Das Plasminogenaktivator-System ist in den Mittelpunkt des Interesses für die Entwicklung tumorreduzierender Substanzen gerückt, denn uPA-abgeleitete Peptide sind nicht nur effektive Inhibitoren der uPA/uPAR-Interaktion, sondern reduzieren nachweislich das Tumorwachstum in vivo. Im Rahmen der Dissertation wurden fünf uPA-abgeleiteten Peptide im neu etablierten Partikelsystem SP-uPA getestet und die dabei erhaltenen IC50-Werte mit den Werten verglichen, die unter nahezu gleichen Bedingungen mit dem Zellsystem U937/FITC-uPA ermittelt wurden. Es wurden keine wesentlichen Unterschiede für beide Systeme festgestellt. SP-uPA stellt ein sehr gut funktionierendes und leicht handhabbares analytisches System zur Untersuchung kompetitiver Substanzen der uPA/uPAR-Bindung dar, das die Vorteile FACS-basierender Messungen vereinigt und gleichzeitig die zeitaufwendige und kostenintensive Kultivierung von im Test einzusetzenden Zellen vermeidet.     «

Das Plasminogenaktivator-System ist in den Mittelpunkt des Interesses für die Entwicklung tumorreduzierender Substanzen gerückt, denn uPA-abgeleitete Peptide sind nicht nur effektive Inhibitoren der uPA/uPAR-Interaktion, sondern reduzieren nachweislich das Tumorwachstum in vivo. Im Rahmen der Dissertation wurden fünf uPA-abgeleiteten Peptide im neu etablierten Partikelsystem SP-uPA getestet und die dabei erhaltenen IC50-Werte mit den Werten verglichen, die unter nahezu gleichen Bedingungen mit d...     »

The plasminogern activator system has become the centre of interest for the development of tumour reducing substances, since uPA derived peptides are not only effective inhibitors of the uPA/uPAR interaction, but also demonstrably reduced tumour growth in vivo. Within the scope of this dissertation five uPA derived peptides were tested in the newly established particle system SP-uPA and the resulting IC 50 values were compared to those determined under almost identical conditions with the U937/FITC-uPA cell system. No significant differences were determined for both systems. SP-uPA represents a very well functioning and easily useable analytical system for the investigation of competing substances for uPA/uPAR binding, which combines the advantages of FACS-based measurements, while at the same time avoiding the time and cost intensive cultivation of cells requiring for testing. The plasminogern activator system has become the centre of interest for the development of tumour reducing substances, since uPA derived peptides are not only effective inhibitors of the uPA/uPAR interaction, but also demonstrably reduced tumour growth in vivo. Within the scope of this dissertation five uPA derived peptides were tested in the newly established particle system SP-uPA and the resulting IC 50 values were compared to those determined under almost identical conditions with the U937/FITC-uPA cell system. No significant differences were determined for both systems. SP-uPA represents a very well functioning and easily useable analytical system for the investigation of competing substances for uPA/uPAR binding, which combines the advantages of FACS-based measurements, while at the same time avoiding the time and cost intensive cultivation of cells requiring for testing.     «

The plasminogern activator system has become the centre of interest for the development of tumour reducing substances, since uPA derived peptides are not only effective inhibitors of the uPA/uPAR interaction, but also demonstrably reduced tumour growth in vivo. Within the scope of this dissertation five uPA derived peptides were tested in the newly established particle system SP-uPA and the resulting IC 50 values were compared to those determined under almost identical conditions with the U937/F...     »