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Title:

Modifying Bacterial Artificial Chromosomes for Extended Genome Modification.

Document type:
Journal Article
Author(s):
Auch, Hannah; Klymiuk, Nikolai; Runa-Vochozkova, Petra
Abstract:
Bacterial artificial chromosomes have been used extensively for the exploration of mammalian genomes. Although novel approaches made their initial function expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to manipulation. Further, protocols for modifying BA...     »
Journal title abbreviation:
Methods Mol Biol
Year:
2022
Journal volume:
2495
Pages contribution:
67-90
Fulltext / DOI:
doi:10.1007/978-1-0716-2301-5_4
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/35696028
Print-ISSN:
1064-3745
TUM Institution:
Professur für Kardiovaskuläre Translation in Großtiermodellen (Prof. Klymiuk)
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