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Titel:

Modifying Bacterial Artificial Chromosomes for Extended Genome Modification.

Dokumenttyp:
Journal Article
Autor(en):
Auch, Hannah; Klymiuk, Nikolai; Runa-Vochozkova, Petra
Abstract:
Bacterial artificial chromosomes have been used extensively for the exploration of mammalian genomes. Although novel approaches made their initial function expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to manipulation. Further, protocols for modifying BA...     »
Zeitschriftentitel:
Methods Mol Biol
Jahr:
2022
Band / Volume:
2495
Seitenangaben Beitrag:
67-90
Volltext / DOI:
doi:10.1007/978-1-0716-2301-5_4
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/35696028
Print-ISSN:
1064-3745
TUM Einrichtung:
Professur für Kardiovaskuläre Translation in Großtiermodellen (Prof. Klymiuk)
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