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- Document type:
- Journal Article; Research Support, Non-U.S. Gov't
- Author(s):
- Pradhan, Arpit Kumar; Shi, Qinfang; Tartler, Katharina Johanna; Rammes, Gerhard
- Title:
- Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q.
- Abstract:
- Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes the treatment of free-floating sections with Aβ peptide and confocal imaging of individual astrocytes. We also provide a comprehensive analysis for 3D rendering of astrocytes and assessment of synapti... »
- Journal title abbreviation:
- STAR Protoc
- Year:
- 2022
- Journal volume:
- 3
- Journal issue:
- 4
- Fulltext / DOI:
- doi:10.1016/j.xpro.2022.101687
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/36125933
- TUM Institution:
- Klinik für Anästhesiologie (DHM)
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