A Modified Burn Comb Model With a New Dorsal Frame That Allows for Local Treatment in Partial-Thickness Burns in Rats.

Weiss, Fabian; Agua, Kariem; Weinzierl, Andrea; Schuldt, Anna; Egana, Jose Tomas; Schlitter, Anna Melissa; Steiger, Katja; Machens, Hans-Günther; Harder, Yves; Schmauss, Daniel

doi:10.1093/jbcr/irac032

2022

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Titel:: A Modified Burn Comb Model With a New Dorsal Frame That Allows for Local Treatment in Partial-Thickness Burns in Rats.
Dokumenttyp:: Journal Article
Autor(en):: Weiss, Fabian; Agua, Kariem; Weinzierl, Andrea; Schuldt, Anna; Egana, Jose Tomas; Schlitter, Anna Melissa; Steiger, Katja; Machens, Hans-Günther; Harder, Yves; Schmauss, Daniel
Abstract:: Burn wound progression (BWP) leads to vertical and horizontal injury extension. The "burn comb model" is commonly used, in which a full-thickness burn with intercalated unburned interspaces is induced. We aimed to establish an injury progressing to the intermediate dermis, allowing repeated wound evaluation. Furthermore, we present a new dorsal frame that enables topical drug application. Eight burn fields and six interspaces were induced on each of 17 rats' dorsa with a 10-second burn comb application. A developed 8-panel aluminum frame was sutured onto 12 animals and combined with an Elizabethan collar. Over 14 days, macroscopic and histologic wound assessment and laser speckle contrast imaging (LSCI) were performed besides evaluation of frame durability. The 10-second group was compared with nine animals injured with a full-thickness 60-second model. Frame durability was sufficient up to day 4 with 8 of the 12 frames (67%) still mounted. The 60-second burn led to an increased extent of interspace necrosis (P = .002). The extent of necrosis increased between days 1 and 2 (P = .001), following the 10-second burn (24% ± SEM 8% to 40% ± SEM 6%) and the 60-second burn (57% ± SEM 6% to 76% ± SEM 4%). Interspace LSCI perfusion was higher than burn field perfusion. It earlier reached baseline levels in the 10-second group (on day 1: 142% ± SEM 9% vs 60% ± SEM 5%; P < .001). Within day 1, the 10-second burn showed histological progression to the intermediate dermis, both in interspaces and burn fields. This burn comb model with its newly developed fixed dorsal frame allows investigation of topical agents to treat BWP in partial-thickness burns.
Zeitschriftentitel:: J Burn Care Res
Jahr:: 2022
Band / Volume:: 43
Heft / Issue:: 6
Seitenangaben Beitrag:: 1329-1336
Volltext / DOI:: doi:10.1093/jbcr/irac032
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/35259276
Print-ISSN:: 1559-047X
TUM Einrichtung:: 597; Institut für Allgemeine Pathologie und Pathologische Anatomie; Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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