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- Title:
Xenogenic induction of adipose tissue and maintenance through pre- and post-conditioning using external volume expansion.
- Document type:
- Journal Article; Research Support, Non-U.S. Gov't
- Author(s):
- Wu, Mengfan; Matar, Dany Y; Yu, Zhen; Karvar, Mehran; Chen, Ziyu; Ng, Brian; Knoedler, Samuel; Darwish, Oliver; Agarwal, Shailesh; Orgill, Dennis P; Panayi, Adriana C
- Abstract:
- External volume expansion (EVE) has been shown to improve fat graft survival. In this study, we investigated the xenogenic implantation of human allograft adipose matrix (AAM) in non-immunocompromised mice in combination with pre- and post-conditioning with EVE to assess long-term adipose tissue survival. Sixty-eight recipient sites in thirty-four eight-week-old wild type (C57BL/6J) mice were separated into four groups. Thirty-four sites received no conditioning and either a subcutaneous injection of 300 μl saline (n= 17; PBS group) or AAM (n= 17; AAM group). Thirty-four sites received pre-conditioning with EVE (Day -7-3 pre-grafting) and 300 μl of AAM. Seventeen of these sites received immediate post-conditioning (Day 1-5 post-grafting) and 17 delayed post-conditioning (Day 28-32 post-grafting). Tissue was harvested at week 12 for analysis. At 12 weeks, immediate and delayed post-conditioning enabled higher volume retention (p= 0.02 andp< 0.0001, respectively). Adipose Stem Cells were greater in the AAM+Del-EVE group compared to the AAM (p= 0.01). Microvessel density was lower in the AAM group compared to the AAM+Imm-EVE (p= 0.04) and AAM+Del-EVE group (p= 0.02). Macrophage infiltration was lower in the AAM+Imm-EVE (p= 0.002) and AAM+Del-EVE (p= 0.003) groups compared to the AAM group. PCR analysis and Western blotting identified a significantly higher expression of PPAR-γ, LPL and VEGF with delayed-conditioning. Pre- and post-conditioning, particularly delayed-post-conditioning, of the recipient site optimized the microenvironment allowing significant adipogenesis and survival of neo-adipose tissue through robust angiogenesis. This study supports that xenogenic transplantation of adipose matrix allows adipose tissue formation and survival with EVE as an adjuvant.
- Journal title abbreviation:
- Biomed Mater
- Year:
- 2022
- Journal volume:
- 17
- Journal issue:
- 6
- Fulltext / DOI:
- doi:10.1088/1748-605X/ac934b
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/36126655
- Print-ISSN:
- 1748-6041
- TUM Institution:
- Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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