Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus.

Zhang, Fangfang; Meier, Anna B; Sinnecker, Daniel; Engelhardt, Stefan; Lipp, Peter; Laugwitz, Karl-Ludwig; Dorn, Tatjana; Moretti, Alessandra

doi:10.1016/j.scr.2022.102785

2022

Back
Back to start of result list
Permanent link for displayed object

Document type:: Journal Article
Author(s):: Zhang, Fangfang; Meier, Anna B; Sinnecker, Daniel; Engelhardt, Stefan; Lipp, Peter; Laugwitz, Karl-Ludwig; Dorn, Tatjana; Moretti, Alessandra
Title:: Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus.
Abstract:: Assessment of the electrophysiological properties of cardiomyocytes is necessary for phenotyping cardiac disorders and for drug screening. Optical action potential imaging using a genetically encoded voltage-sensing fluorescent protein (VSFP) allows for high-throughput functional characterization of cardiomyocytes, which offers an advantage over the traditional patch-clamp technique. Here, we knocked VSFP into the AAVS1 safe harbor locus of human iPSCs, generating two stable voltage indicator lines - one heterozygous (MRIi003-A-5) and the other homozygous (MRI003-A-6). Both lines can be used for optical membrane potential recordings and provide a powerful platform for a wide range of applications in cardiovascular biomedicine. «
Assessment of the electrophysiological properties of cardiomyocytes is necessary for phenotyping cardiac disorders and for drug screening. Optical action potential imaging using a genetically encoded voltage-sensing fluorescent protein (VSFP) allows for high-throughput functional characterization of cardiomyocytes, which offers an advantage over the traditional patch-clamp technique. Here, we knocked VSFP into the AAVS1 safe harbor locus of human iPSCs, generating two stable voltage indicator li... »
Journal title abbreviation:: Stem Cell Res
Year:: 2022
Journal volume:: 61
Fulltext / DOI:: doi:10.1016/j.scr.2022.102785
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/35421847
Print-ISSN:: 1873-5061
TUM Institution:: Institut für Pharmakologie und Toxikologie; Klinik und Poliklinik für Innere Medizin I, Kardiologie; Professur für Regenerative Medizin bei Kardiovaskulären Erkrankungen (Prof. Moretti)
BibTeX