Raman spectroscopy is a promising tool for the analysis of bacteria. It provides high molecular information at microscopic resolution. In this work a protocol to measure the metabolic activity of bacteria by means of deuterium uptake using D₂O-containing medium was developed. Thus rapid antibiotic susceptibility testing in particular of heteroresistant bacteria was possible. Likewise, the so-called dormant state of Mycobacterium smegmatis was determined by means of cluster analysis of Raman spectra.     «

Raman spectroscopy is a promising tool for the analysis of bacteria. It provides high molecular information at microscopic resolution. In this work a protocol to measure the metabolic activity of bacteria by means of deuterium uptake using D₂O-containing medium was developed. Thus rapid antibiotic susceptibility testing in particular of heteroresistant bacteria was possible. Likewise, the so-called dormant state of Mycobacterium smegmatis was determined by means of cluster analysis of Raman spec...     »