Stereomicroscopic and differential interference contrast (DIC) images of 48 h submerged cultivations of the filamentous fungus Aspergillus niger

The dataset provides 2694 stereomicroscopic 8-bit RGB JPEG images of 44 sampling times from two 48 h submerged cultivations of the filamentous fungus Aspergillus niger. The cultivations were performed in shake flasks with identical cultivation parameters. About 1300 images were taken for each cultivation flask. At least 24 images per culture and sampling time were taken. The stereomicroscopic images depict the development of Aspergillus niger pellets starting from the agglomeration of spores after inoculation followed by spore germination and outgrowth into a pellet population. The following pixel sizes and field of views (FoV) were used at the different sampling times: 0 h – 1.5 h, pixel size 0.82 µm, FoV 1.49 mm × 1.12 mm; 2 h – 2.5 h, pixel size 1.63 µm, FoV 2.97 mm × 2.22 mm; 3 h – 3.5 h, pixel size 3.33 µm, FoV 6.07 mm × 4.55 mm; 4 h – 48 h, pixel size 4.08 µm, FoV 7.44 mm × 5.58 mm. Further, the dataset provides 1144 DIC 8-bit grayscale TIFF images of 44 sampling times from the same cultivations. 20 to 40 images per sampling time with 100 and 400 fold magnification resulting in pixel sizes of 0.65 µm and 0.16 µm, respectively, were taken.      «

The dataset provides 2694 stereomicroscopic 8-bit RGB JPEG images of 44 sampling times from two 48 h submerged cultivations of the filamentous fungus Aspergillus niger. The cultivations were performed in shake flasks with identical cultivation parameters. About 1300 images were taken for each cultivation flask. At least 24 images per culture and sampling time were taken. The stereomicroscopic images depict the development of Aspergillus niger pellets starting from the agglomeration of spores aft...     »

The stereomicroscopic imaging was performed with a Leica S8APO stereomicroscope (Leica Microsystems GmbH, Wetzlar, Germany) connected to a Leica MC120 HD camera (Leica Microsystems GmbH, Wetzlar, Germany). For image capture, 3 mL of culture volume were transferred into a petri dish (Sarstedt AG & Co. KG) and diluted with distilled H2O. The petri dish was put on a blue cardboard with a rectangular grid in the measurement area of the stereo microscope. One image per 13 × 11 mm rectangle were taken. The DIC imaging was performed with a Leica DM 5000 CS (Leica Microsystems GmbH, Wetzlar, Germany). For image capture, 15 µl of culture volume were transferred on a microscope slide and covered with a cover glass.      «

The stereomicroscopic imaging was performed with a Leica S8APO stereomicroscope (Leica Microsystems GmbH, Wetzlar, Germany) connected to a Leica MC120 HD camera (Leica Microsystems GmbH, Wetzlar, Germany). For image capture, 3 mL of culture volume were transferred into a petri dish (Sarstedt AG & Co. KG) and diluted with distilled H2O. The petri dish was put on a blue cardboard with a rectangular grid in the measurement area of the stereo microscope. One image per 13 × 11 mm rectangle were taken...     »