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Title:

Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica

Document type:
Zeitschriftenaufsatz
Author(s):
Rauwolf S, Steegmüller T, Schwaminger SP, Berensmeier S:
Abstract:
Silica is widely used for chromatography resins due to its high mechanical strength, column efficiency, easy manufacturing (i.e. controlled size and porosity), and low-cost. Despite these positive attributes to silica, it is currently used as a backbone for chromatographic resins in biotechnological downstream processing. The aim of this study is to show how the octapeptide (RH)4 can be used as peptide tag for high-purity protein purification on bare silica. The tag possesses a high affinity to...     »
Journal title:
Engineering in Life Sciences
Year:
2021
Year / month:
2021-06
Covered by:
Scopus
Reviewed:
ja
Language:
en
Fulltext / DOI:
doi:10.1002/elsc.202100019
WWW:
https://onlinelibrary.wiley.com/doi/full/10.1002/elsc.202100019
Date of publication:
04.06.2021
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