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- Dokumenttyp:
- Journal Article
- Autor(en):
- Keller, Sarah; Borde, Tabea; Brangsch, Julia; Reimann, Carolin; Kader, Avan; Schulze, Daniel; Buchholz, Rebecca; Kaufmann, Jan O; Karst, Uwe; Schellenberger, Eyk; Hamm, Bernd; Makowski, Marcus R
- Titel:
- Assessment of the hepatic tumor extracellular matrix using elastin-specific molecular magnetic resonance imaging in an experimental rabbit cancer model.
- Abstract:
- To investigate the imaging performance of an elastin-specific molecular magnetic resonance imaging (MRI) probe with respect to the extracellular matrix (ECM) in an experimental hepatic cancer model. Twelve rabbits with hepatic VX2 tumors were examined using 3 T MRI 14, 21, and 28 days after tumor implantation for two subsequent days (gadobutrol, day 1; elastin-specific probe, day 2). The relative enhancement (RE) of segmented tumor regions (central and margin) and the peritumoral matrix was calculated using pre-contrast and delayed-phase T1w sequences. MRI measurements were correlated to histopathology and element-specific and spatially resolved mass spectrometry (MS). Mixed-model analysis was performed to assess the performance of the elastin-specific probe. In comparison to gadobutrol, the elastin probe showed significantly stronger RE, which was pronounced in the tumor margin (day 14-28: P ≤ 0.007). In addition, the elastin probe was superior in discriminating between tumor regions (χ2(4) = 65.87; P < 0.001). MRI-based measurements of the elastin probe significantly correlated with the ex vivo elastinstain (R = .84; P <0 .001) and absolute gadolinium concentrations (ICP-MS: R = .73, P <0 .01). LA-ICP-MS imaging confirmed the colocalization of the elastin-specific probe with elastic fibers. Elastin-specific molecular MRI is superior to non-specific gadolinium-based contrast agents in imaging the ECM of hepatic tumors and the peritumoral tissue.
- Zeitschriftentitel:
- Sci Rep
- Jahr:
- 2020
- Band / Volume:
- 10
- Heft / Issue:
- 1
- Volltext / DOI:
- doi:10.1038/s41598-020-77624-8
- PubMed:
- http://view.ncbi.nlm.nih.gov/pubmed/33247185
- Print-ISSN:
- 2045-2322
- TUM Einrichtung:
- Institut für Diagnostische und Interventionelle Radiologie
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