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Titel:

Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines.

Dokumenttyp:
Journal Article
Autor(en):
Zhang, Fangfang; Meier, Anna B; Laugwitz, Karl-Ludwig; Dorn, Tatjana; Moretti, Alessandra
Abstract:
Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines - one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) - by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential an...     »
Zeitschriftentitel:
Stem Cell Res
Jahr:
2021
Band / Volume:
57
Volltext / DOI:
doi:10.1016/j.scr.2021.102612
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/34864222
Print-ISSN:
1873-5061
TUM Einrichtung:
Arbeitsgruppe Tissue Engineering und Regenerative Medizin; Klinik und Poliklinik für Innere Medizin I, Kardiologie
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