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Title:

cDNA-directed expression of human cytochrome P450 CYP1A1 using baculovirus. Purification, dependency on NADPH-P450 oxidoreductase, and reconstitution of catalytic properties without purification.

Document type:
Journal Article
Author(s):
Buters, J T; Shou, M; Hardwick, J P; Korzekwa, K R; Gonzalez, F J
Abstract:
A recombinant baculovirus containing the human cytochrome P450 (CYP) 1A1 cDNA was constructed and used to express CYP1A1 in Spodoptera frugiperda (SF9) insect cells (0.14 +/- 0.04 nmol/mg protein, 53 +/- 14 nmol/liter, N = 30). The enzyme represented approximately 1% of total cellular protein and was partially purified by a three-column procedure to a specific content of 5.0 nmol/mg protein. Catalytic activity was reconstituted with both the purified enzyme using lipid and NADPH-P450 oxidoreduct...     »
Journal title abbreviation:
Drug Metab Dispos
Year:
1995
Journal volume:
23
Journal issue:
7
Pages contribution:
696-701
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/7587956
Print-ISSN:
0090-9556
TUM Institution:
Molekulare Allergologie (Prof. Schmidt-Weber)
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