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Titel:

Cytochrome P450 2A1, 2E1, and 2C9 cDNA-expression by insect cells and partial purification using hydrophobic chromatography.

Dokumenttyp:
Journal Article
Autor(en):
Grogan, J; Shou, M; Andrusiak, E A; Tamura, S; Buters, J T; Gonzalez, F J; Korzekwa, K R
Abstract:
High-level expression of three cloned cytochrome P450 enzymes was accomplished using the baculovirus-insect cell expression system. The amount of enzyme expression was enhanced by cell infections in the presence of medium-supplements containing hemin and by growth in suspension cultures. Human cytochromes P450 2E1 and 2C9 and rat cytochrome P450 2A1 were partially purified from cell extracts using hydrophobic interaction and hydroxyapatite chromatography. The resulting enzymes were of estimated...     »
Zeitschriftentitel:
Biochem Pharmacol
Jahr:
1995
Band / Volume:
50
Heft / Issue:
9
Seitenangaben Beitrag:
1509-15
Volltext / DOI:
doi:10.1016/0006-2952(95)02065-9
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/7503803
Print-ISSN:
0006-2952
TUM Einrichtung:
Molekulare Allergologie (Prof. Schmidt-Weber)
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